Composite

Part:BBa_J36845:Design

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-30)


Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 953
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 962
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 383
    Illegal AgeI site found at 917
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.


Source

Assembly of J36835, J36836, J36843

References